Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2017 , Vol 21 , Issue 2
RP-HPLC Estimation of Alogliptin and Pioglitazone Simultaneously in Combined Tablet Dosage Forms
B. Haribabu1,P. Rama Krishna Veni2,K. Bala Murali Krishna1,K. Lakshmi Prameela3
1Department of Chemistry, Acharya Nagarjuna University, Nagarjuna nagar, Guntur-522510, Andhra Pradesh, India
2Department of Applied Sciences and Humanities, Sasi Institute of Technology and Engineering, Tadepalligudem - 534101, Andhra Pradesh, India
3Department of Chemistry, SGK Government Degree College, Vinukonda-522647, Andhra Pradesh, India
DOI : 10.12991/marupj.300864 A high performance liquid chromatographic method was developed to quantify alogliptin and pioglitazone simultaneously in bulk and combined tablet dosage form. The chromatographic analysis was done on a Zorbax C8 column (150 mm x 4.6 mm internal diameter, 5 μm particle size) with a mobile phase of 0.1 M ammonium acetate and methanol (50:50, v/v) at 1.0 mL/min. The effluents were monitored at 248 nm and the retention time of alogliptin and pioglitazone were 2.883 min and 4.329 min, respectively. Calibration curves were linear from 6.25-18.75 μg/mL for alogliptin and 11.25-33.75 μg/mL for pioglitazone. The LOD and LOQ values for alogliptin were 0.047 and 0.157 μg/mL, respectively; corresponding values for pioglitazone were 0.085 and 0.284 μg/ mL, respectively. The precision for alogliptin and pioglitazone was in the range of 0.094-0.303% and 0.072-0.239%, respectively, with corresponding accuracy of 99.450-99.692% and 100.184-100.422%. The developed and validated method was successfully applied for the simultaneous determination of alogliptin and pioglitazone in tablet formulation. Keywords : Alogliptin, Pioglitazone, Liquid Chromatography, Tablets, Assay
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