Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2018 , Vol 22 , Issue 1
Comparative phytochemical evaluation of natural and micropropagated plants of Bacopa monnieri (L.)
Firoj A. TAMBOLI1,Vinod D. RANGARI2,Suresh G. KILLEDAR1,Sagar U. JADHAV1,Trupti S. GHATAGE3,Vishwajeet P. KORE1
1Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Kolhapur (M.S.) India
2Department of Pharmacognosy, S.L.T. Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya (Central University), Koni, Bilaspur – 495009 (CG) India
3Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, India
DOI : 10.12991/mpj.2018.42 Bacopa monnieri Linn. an important medicinal plant of Scrophulariaceae family and is distributed in the wet and marshy lands throughout India, Nepal, Sri Lanka, China, Taiwan, and Hawaii. It is endangered species so it is necessary to preserve germplasm. The objective of this research work is the comparative phytochemical evaluation of natural and micro-propagated plants of Bacopa monnieri (BM). It shows the presence of various phytoconstituents namely, alkaloids, saponins, phenolics, and flavonoids. These phytoconstituents are responsible for anti-oxidant and various other activity of the plant. The protocols for tissue culture has been developed for regeneration of the BM plant from small part (nodal segment). The regenerated plant having the similar potential in terms of phytoconstituents. The protocols of Murashige and Skoog medium can be used for the clonal regeneration of plant on the large scale through which it can be protected and preserved for the long duration in small space. From the comparative phytochemical investigation by HPTLC, the phytoconstituents which occur in the micro propagated plant are similar to that of the natural plant of B. monnieri. Keywords : Bacopa monnieri Linn., flavonoids, HPTLC, Micropropagation, Brahmi
Marmara University