ACTA Pharmaceutica Sciencia 2018 , Vol 56 , Num 1
Investigation of the Anti-inflammatory, Hypoglycemic Activity and Median Lethal Dose (LD50) Level of Limonene in Mice and Rats
Betül Sever Yılmaz 1 Hanefi Özbek 2
1 Ankara University, School of Pharmacy, Department of Pharmacognosy, Ankara, Turkey
2 Istanbul Medipol University, School of Medicine, Department of Medical Pharmacology, Istanbul, Turkey
DOI : 10.23893/1307-2080.APS.05606 The aim of this study is to investigate the anti-inflammatory, hypoglycemic activity and median lethal lose (LD50) level of limonene mice and rats.

Lethal dose levels were investigated using the probit analysis method. For the measurement of anti-inflammatory activity, seven separate work groups were established and limonene was administered in three different doses 0.15, 0.30, 0.60 mL/ kg. Indomethacin and etodolac were used as reference anti-inflammatory agents.

For the evaluation of hypoglycemic activity, 6 separate work groups, consisting of healthy and diabetic mice were established and glibenclamide was used as the reference agent.

The LD50 level of limonene was determined to be 2.77 mL/kg. It is determined that all administered dosages of limonene possess anti-inflammatory activity; among these, 0.30 mL/kg was equivalent to indomethacin, and the remaining dosages were equivalent to etodolac. No hypoglycemic activity of limonene was observed in healthy and diabetic mice.

As a consequence, it is concluded that limonene did not show hypoglycemic activity, but possessed a strong anti-inflammatory activity. Keywords : Limonene, anti-inflammatory activity, hypoglycemic activity, median lethal dose, rats, mice

Istanbul Medipol University