¹Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey
²Department of Pharmacognosy, Faculty of Pharmacy, Eastern Mediterranean University, Gazimagusa, Turkish Republic of Northern Cyprus-via Mersin 10 Turkey
³Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey
⁴Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey DOI : 10.12991/jrp.2018.80 A rapid and simple high-performance liquid chromatography method with a Photo Diode Array (PDA) detector is developed for the simultaneous analysis of seven kaempferol, quercetin and myricetin derivatives along with an acylated flavonoid glycoside; trans-tiliroside from different Cistus species. In this study, the qualitative and quantitative analysis of the methanolic extracts of three Cistus species (C. creticus L., C. laurifolius L. and C. salviifolius L.) growing in Anatolia in terms of characterization of flavonoid compounds were performed by RP-HPLC for the first time. Trans-tiliroside, a mono-coumaroyl kaempferol glucoside was found to be the most abundant flavonoid in C. salviifolius (0.276±0.003 g/100 gextract) and C. creticus (0.253±0.001 g/100 gextract) while hyperin (0.149±0.004 g/100 gextract) and myricetin 3-O-β-galactopyranoside (0.139±0.006 g/100 gextract) were found to be the most intense flavonoids in C. laurifolius samples. The described HPLC method appears suitable for the determination of the Cistus flavonols and their glycosides and can be considered as an effective and alternative procedure for the identification and quantification of this important class of biologically active compounds. Keywords : Cistus; Cistaceae; flavonols; HPLC-PDA