¹Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Kolhapur (M.S.) India
²Department of Pharmacognosy, S.L.T. Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya (Central University), Koni, Bilaspur – 495009 (CG) India
³Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, India DOI : 10.12991/mpj.2018.42 Bacopa monnieri Linn. an important medicinal plant of Scrophulariaceae family and is distributed in the wet and marshy lands throughout India, Nepal, Sri Lanka, China, Taiwan, and Hawaii. It is endangered species so it is necessary to preserve germplasm. The objective of this research work is the comparative phytochemical evaluation of natural and micro-propagated plants of Bacopa monnieri (BM). It shows the presence of various phytoconstituents namely, alkaloids, saponins, phenolics, and flavonoids. These phytoconstituents are responsible for anti-oxidant and various other activity of the plant. The protocols for tissue culture has been developed for regeneration of the BM plant from small part (nodal segment). The regenerated plant having the similar potential in terms of phytoconstituents. The protocols of Murashige and Skoog medium can be used for the clonal regeneration of plant on the large scale through which it can be protected and preserved for the long duration in small space. From the comparative phytochemical investigation by HPTLC, the phytoconstituents which occur in the micro propagated plant are similar to that of the natural plant of B. monnieri. Keywords : Bacopa monnieri Linn., flavonoids, HPTLC, Micropropagation, Brahmi