Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2021 , Vol 25 , Issue 1
Bioactivity-guided fractionation of a methanol leaf extract from Gnetum africanum with potential anti-diabetic activity: (-)-Epicatechin as the active principle
Nkeiruka Emmanuela UDEH1,Charles Okeke NNADI2,Aruh Otah ANAGA3,Isaac Uzoma ASUZU3
1Department of Veterinary Biochemistry and Animal Production, College of Veterinary Medicine, Michael Okpara University of Agriculture, Umudike PMB 7267, Umuahia, Abia State, Nigeria
2Department of Pharmaceutical and Medicinal Chemistry, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria
3Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria
DOI : 10.35333/jrp.2021.293 Dietary constituents of plants such as flavonoids are very important in ameliorating the challenges of metabolic disorders such as diabetes mellitus. The study evaluated the antidiabetic activities of fractions and a known flavonoid isolated from Gnetum africanum (Welw) of fasting blood sugar (FBS) in alloxan-induced diabetic albino rats. Antidiabetic activity-guided isolation by column chromatographic (CC) separation of methanol extract and purification of the most active CC fractions by semi-preparative high performance liquid chromatograpgy (HPLC) yielded a known flavonoid (GAF7.4) and four other uncharacterized fractions. The structure of GAF7.4 was elucidated based on the 1D and 2D NMR and HREIMS spectroscopic analyses. Antidiabetic activity was conducted by alloxan-induced FBS in diabetic rats model using glibenclamide as standard. The flavonoid (GAF7.4) was identified as (-)-epicatechin. The CC fraction 7 (50 mg/kg) elicited significant (p < 0.05) reduction in FBS of 42.3 % after 6 h. The isolated flavonoid (GAF7.4), 10 mg/kg dose caused a significantly higher reduction in FBS of 71.4 % in alloxan induced diabetic rats compared with 41.2 % reduction in glibenclamide (2 mg/kg) control. This represented the first report of epicatechin in G. africanum and our findings have contributed new knowledge to antidiabetic constitutents of the plants. Keywords : Gnetum africanum; alloxan; fasting blood sugar; (-)-epicatechin; antidiabetic
Marmara University