Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2019 , Vol 23 , Issue 6
Cochlear electrophysiology and histopathology of rats exposed to intratympanic silica nanoparticles
Erkan ÖZBAY1,Ülkü ÇÖMELEKOĞLU2,Yusuf VAYISOGLU3,Onder ALBAYRAK4,Rabia BOZDOGAN ARPACI5,Burcu DEMIRBAG6,Döndü Anış DENİZ3,Derya Umit TALAS3
1Vocational School of Health Service, Karamanoğlu Mehmetbey University, Karaman, Turkey
2Department of Biophysics, Faculty of Medicine, Mersin University, Mersin, Turkey
3Department of Otorhinolaryngology, Faculty of Medicine, Mersin University, Mersin, Turkey
4Department of Mechanical Engineering, Faculty of Engineering, Mersin University, Mersin, Turkey
5Department of Pathology, Mersin University, Faculty of Medicine, Mersin, Turkey
6Department of Histology and Embryology, Mersin University, Faculty of Medicine, Mersin, Turkey
DOI : 10.35333/jrp.2019.76 Toxic effects of silica nanoparticle of rats exposed intratympanically for 7 days were investigated using brainstem auditory evoked potential and distortion product otoacoustic emission techniques. The histopathological changes were evaluated with light microscopy and field emission-scanning electron microscopy were demonstrated in cochleas using based energy-dispersive X-ray spectroscopy. The reduction in the amplitude and prolongation at the peak latency and interpeak latencies of brainstem auditory evoked potential were observed. Silica nanoparticles significantly reduced distortion product otoacoustic emission amplitude in the 2, 3 and 4 kHz frequencies. In the light microscopic evaluation, the samples obtained from the experimental group showed a significant loss of hair cells. Field emission scanning electron microscope analysis of experimental group showed considerable hair cell damage compared to control group. The findings of this study show that intratympanic administration of silica nanoparticles may lead to hearing impairment by causing structural changes in cochlear hair cells. Keywords : Silica nanoparticle; cochlea; brainstem auditory potential; otoacoustic emission; hair cell; histopathology
Marmara University