Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2019 , Vol 23 , Issue 3
Effects of different sizes silica nanoparticle on the liver, kidney and brain in rats: Biochemical and histopathological evaluation
Ülkü ÇÖMELEKOĞLU1,Ebru BALLI 2,Serap YALIN3,Pelin EROĞLU4,Gülsen BAYRAK2,Selma YAMAN5,Fatma SÖĞÜT6
1Mersin University, Faculty of Medicine, Department of Biophysics, Mersin, Turkey
2Mersin University, Faculty of Medicine, Department of Histology and Embryology, Mersin, Turkey
3Mersin University, Faculty of Pharmacy, Department of Biochemistry, Mersin, Turkey
4Mersin University, Faculty of Science and Literature, Department of Chemistry, Mersin, Turkey
5Kahramanmaraş Sütçü İmam University, Faculty of Medicine, Department of Biophysics, Kahramanmaraş, Turkey
6Mersin University, Vocational School of Medical Services, Department of Perfusion Technology, Mersin, Turkey
DOI : 10.12991/jrp.2019.142 Silica is among the most popular nanoparticles. Large-scale production and use have increased the risk of human exposure to silica nanoparticles (SiO2NPs). This study aimed to investigate the toxic effects on the kidney, liver and brain of the SiO2NPs. Twenty eight male Wistar albino rats were divided into four groups (n=7 rats) as control (1 mL/day physiological saline administration for 28 day), 6 nm SiO2NP (6 nm, 150 μg/mL/day for 28 day), 20 nm SiO2NP (20 nm, 150 μg/mL/day for 28 day) and 50 nm SiO2NP (50 nm, 150 μg/mL/day for 28 day) groups. After the last administration, rats were sacrificed and kidney, liver and brain samples were taken for biochemical and histological investigation. In all groups, malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activities were measured using the spectrophotometric methods. Ultrastructural changes of tissues were evaluated using transmission electron microscopy. In the kidney tissue, the MDA level significantly increased in the 6 and 50 nm groups, while the similar increase was observed in the 6 nm group of the liver and 20 and 50 nm groups of the brain. SOD activity significantly increased in the 6, 20 and 50 nm groups in brain and kidney tissues, but no significant change was observed in the liver tissue groups. Catalase activity decreased in the kidneys at 6 and 50 nm groups and increased in the 20 and 50 nm groups in liver and brain tissues. Ultrastructurally, kidney and liver tissues had normal morphological features in all groups. Degenerative changes were observed in the nerve fibers and axoplasm of myelinated and unmyelinated nerve fibers in 6 nM, 20 nM and 50 nM SiO2NP groups in the brain. These findings showed that exposure to 6, 20 and 50 nm sizes SiO2 NPs may cause toxic effects in the liver, kidney and brain. Keywords : Silica nanoparticle; oxidative stress; kidney; liver; brain; histopathology
Marmara University