Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2019 , Vol 23 , Issue 2
Biological activity and chemical composition of the essential oil of Nepeta cataria L.
Behnam ASHRAFI1,Parvin RAMAK2,Behrouz EZATPOUR3,Gholam Reza TALEI3
1Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
2Research Division of Natural Resources, Lorestan Agricultural and Natural Resources Research and Education Center, AREEO, Khorramabad, Iran
3Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
DOI : 10.12991/jrp.2019.141 In this study, chemical composition, antioxidant and antimicrobial activity of the essential oil of Nepeta cataria L., a native plant that grows in Lorestan region of Iran, were studied. Chemical constituents of the essential oil were identified by means of gas chromatography/mass spectrometry (GC/MS) and the relative content of each constituent was determined by area normalization. Disc diffusion and broth microdilution assays were used for in vitro antimicrobial screening. Antioxidant activity was employed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging. The predominant bioactive ingredients with high percentage in N. Cataria essential oil was 4a-α, 7-α, 7a-β-Nepetalactone (53.87%). The essential oil’s IC50 value of 80.62 μg / mL in the DPPH assay, could be regarded as its strong antioxidant potential. The antibacterial activities of the essential oil were screened and compared against dozen important human pathogens. The essential oil showed good antibacterial activity, especially on Salmonella spp.. The biggest diameters of inhibition zones (29 mm) was recorded for Salmonella typhi and Salmonella paratyphi A showed the lowest MIC (160 μg / mL). Keywords : Antimicrobial; antioxidant, Nepeta cataria L.; essential oil
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