Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2018 , Vol 22 , Issue 1
Determination of antioxidant activities of solvent extracts from an endemic plant: Phlomis leucophracta
Cengiz Sarıkürkçü1,Mustafa Cengiz2,Ahmet Küçükyumru2,Gökhan Zengin3
1Süleyman Demirel University, Faculty of Pharmacy, Department of Analytical Chemistry, Isparta, Turkey
2Süleyman Demirel University, Faculty of Science and Literature, Department of Chemistry, Isparta, Turkey
3Selcuk University, Faculty of Science, Department of Biology, Konya, Turkey
DOI : 10.12991/mpj.2018.45 The members of the genus Phlomis have been traditionally used for therapeutic purposes in Turkey. In this study, the antioxidant properties of different extracts from P. leucophracta were investigated. Antioxidant properties were evaluated by different assays including free radical scavenging (DPPH assay), reducing power (potassium ferricyanide method), β-carotene/linoleic acid, metal chelating and phosphomolybdenum. Moreover, total phenolic and flavonoid contents were detected for each extracts. Total phenolic and flavonoid contents were detected as 30.86-55.00 mg GAE/g extract and 4.93-26.09 mg QE/g extract, respectively. The methanol and water extracts exhibited higher DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging and reducing power abilities as compared to ethyl acetate and hexane extracts. The best activity was observed by the hexane extract in β-carotene/linoleic acid assay (94.35% at 2 mg/mL). In metal chelating ability, those samples exhibited the following order (at 0.25 mg/mL concentration): Water (73.90%)>Hexane(64.87%)>Ethyl acetate(4.88%)>Methanol (2.28%). Based on our results, P. leucophracta may be utilized as a natural source of antioxidant compounds in food and pharmaceutical areas. Keywords : Phlomis leucophracta; phenolics; DPPH; antioxidant activity; reducing power
Marmara University