Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2016 , Vol 20 , Issue 3
In vitro PDGF-B gene silencing studies and In vivo delivery of siRNA to the rat kidney using Chitosan/siRNA nanoplexes
Emine SALVA1, Suna OZBAS TURAN2, Saadet ALAN3, Julide AKBUGA2
1İnönü University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Malatya, Turkey
2Marmara University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Haydarpaşa-İstanbul, Turkey
3İnönü University, Faculty of Medicine, Department of Medical Pathology, Malatya, Turkey
DOI : 10.12991/mpj.20162082721 The targeting of specific genes responsible from onset and progression of kidney diseases offer a new therapeutic strategy in the field of renal gene therapy. The altered expression of platelet derived growth factor (PDGF) is an important marker of renal diseases. In this study, we investigated in vitro gene silencing efficiency of chitosan nanoplexes containing PDGF-B and PDGFR-β targeted siRNAs in the kidney cell lines including HEK-293 and MDCK and delivery to the kidney as an in vivo delivery system. As a result, PDGF-B expression was significantly inhibited by co-delivery of chitosan/siPDGFB+ siPDGFR-β nanoplexes prepared using in the different weight ratios (10/1, 20/1 and 50/1). When 20/1 and 50/1 weight ratios of chitosan nanoplexes were i.v. injected to rats, chitosan/ FITC-siPDGFB nanoplexes were reached to kidney tissue at 4 h after intravenous injection. These results suggest that delivery of siRNA using chitosan nanoplexes may be effective for the therapy of kidney diseases. Keywords : siRNA, PDGF-B, PDGFR-β, chitosan, nanoplexes, kidney
Marmara University