Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2016 , Vol 20 , Issue 2
In situ production of cationic lipid coated magnetic nanoparticles in multiple emulsions for gene delivery
Hasan AKBABA1, Yusuf SELAMET2, Ayse Gulten KANTARCI3
1Faculty of Pharmacy, Ege University, 35100, İzmir, Turkey
2Faculty of Science, Izmir Institute of Technology, 35433, İzmir, Turkey
3Faculty of Pharmacy, Ege University, 35100, İzmir, Turkey
DOI : 10.12991/mpj.20162069254 Magnetic nanoparticles are effective delivery systems to target therapeutic genes by the attractive forces of magnetic fields. Curative effects depending on dose of nucleic acids or drugs increased, while cytotoxic effects minimized with these systems. In this study, a novel magnetic nanoparticle synthesis method was developed by combining advantages of microemulsion and multiple emulsion methods. Particle size, zeta potential, magnetization, complex formation with nucleic acids, DNase I protection ability, and cytotoxicity levels were examined. At last, magnetic nanoparticles were obtained with a promising synthesis method and it is determined that they are sufficiently small, non-toxic and have optimal surface properties for systemic delivery of nucleic acids. Keywords : Iron oxide, superparamagnetic, cationic magnetic nanoparticle, multiple emulsion, gene delivery, magnetofection
Marmara University