Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2014 , Vol 18 , Issue 1
In vitro investigation of the effects of colistin alone with and tigecycline, imipenem and rifampicin combinations on polymorphonuclear leukocyte functions, oxidative stress, oxidant and antioxidant enzymes in patients with bronchiectasis
Burcak GURBUZ1, Umran SOYOGUL GURER1, Ozge CEVIK2, Irfan YALCINKAYA3, Gulnaz Nural BEKIROGLU4, Adile CEVIKBAS1
1Marmara Üniversitesi Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
2Cumhuriyet Üniversitesi Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Sivas, Türkiye
3Sağlık Bakanlığı , Süreyyapaşa Göğüs Hastalıkları ve Göğüs Cerrahisi Eğitim ve Araştırma Hastanesi, İstanbul, Türkiye
4Marmara Üniversitesi Tıp Fakültesi, Biyoistatistik ve Tıbbi Bilişim Anabilim Dalı, İstanbul, Türkiye
DOI : 10.12991/201414123 In our study was investigated the in vitro effects of Colistin(4 μg/ml), alone with the combination of tigecycline( 0.87 μg/ml), imipenem (30 μg/ml) and rifampicin (7 μg/ml) at therapeutic concentrations and malondialdehyde (MDA) level, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and myeloperoxidase (MPO) activity on polymorphonuclear leukocyte (PMN) functions (phagocytic and intracellular killing activity) of patients with bronchiectasis. PMNLs were isolated from venous blood by Ficoll-hypaque gradient centrifugation method. Phagocytic activity and intracellular killing activity were assayed by modified Alexander"s method. MDA levels were determinated by Beuge"s method, SOD, GSH-Px and MPO activities were assayed by Sun"s, Paglia and Valentine"s and Hillegas"s, methods respectively. All results are statistically evaluated. Phagocytic (p<0.0001), and intracellular killing activity (p=0.0113, p=0.0008, p=0.0014, p=0.0036)in the PMN of patients with bronchiectasis significantly increased when compared to the control. Enzyme activity (SOD, GSH-Px and MPO) and MDA level significantly decreased (p<0.001) in patients with bronchiectasis when compared to the control. Keywords : Antioxidants, bronchiectasis, phagocytosis, oxidative stres
Marmara University