Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2012 , Vol 16 , Issue 1
Synthesis and evaluation of cytotoxic activities of some 1,4-disubstituted thiosemicarbazides, 2,5-disubstituted-1,3,4-thiadiazoles and 1,2,4-triazole-5-thiones derived from benzilic acid hydrazide
Işıl Çoruh1, Sevim Rollas1, Suna Özbaş Turan2, Jülide Akbuğa2
1Marmara Üniversitesi, Eczacılık Fakültesi, Farmasötik Kimya, İstanbul, Türkiye
22Marmara Üniversitesi, Eczacılık Fakültesi, Farmasötik Biyoteknoloji, Istanbul, Türkiye
DOI : 10.12991/201216421 This study describes the synthesis of novel 1-(α,α-diphenyl-α-hydroxy)acetyl-4- substitutedthiosemicarbazide (2a-k), [5-(substitutedamino)-1,3,4-thiadiazole-2-yl](diphenyl) methanol (3a-b) and 3-[hydroxy(diphenyl)methyl]-4-(nonsusbtituted/substituted)-2,4-dihydro- 5H-1,2,4-triazole-5-thione derivatives (4a-c) and evaluation of their cytotoxic activities. In the course of the syntheses benzilic acid methyl ester was reacted with hydrazine hydrate in absolute ethanol to afford benzilic acid hydrazide (1). Reaction of 1 with appropriate alkyl/ arylisothiocyanates gave 1-(α,α-diphenyl-α-hydroxy)acetyl-4-substitutedthiosemicarbazide (2a-k). [5-(substitutedamino)-1,3,4-thiadiazole-2-yl](diphenyl)methanol derivatives (3a-b) were obtained by cyclization of 2a and 2c with concentrated sulphuric acid. On the other hand, 3-[hydroxy(diphenyl)methyl]-4-(nonsusbtituted/substituted)-2,4-dihydro-5H-1,2,4-triazole- 5-thione (4a-c) were obtained by cyclization of 2c, 2d and 2g with 2N NaOH. The structures of the new compounds were confirmed by the data obtained from elemental analysis, HPLC, UV, IR, 1H-NMR, 13C-NMR, HSQC and MS spectra. Compounds 2a, 2c-k, 3b and 4b were selected for cytotoxic screening by using HEK293 cell line of MTT assay. The highest inhibition were confirmed as 50.23% at 10 mg/ml for the compound 1-(α,α-diphenyl-α- hydroxy)acetyl-4-cyclohexylmethylthiosemicarbazide (2e). Keywords : thiosemicarbazide, thiadiazole, 1,2,4-triazole-5-thione, cytotoxic activity
Marmara University