¹National Reference Control Laboratory of Veterinary Drug Residues in product of animal origin and food additives, State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
²Department of Technology of Biologically Active Substances, Pharmacy and Biotechnology, Lviv Polytechnic National University, Lviv, Ukraine
³Laboratory of biochemistry adaptation and ontogenesis of animals, Institute of Animal Biology, Lviv, Ukraine
⁴Faculty of Biology, Ivan Franko National University of Lviv, Ukraine DOI : 10.29228/jrp.17 The study aimed to investigate the effect of Humilid, a biologically active food supplement, on biochemical and hematologic parameters in the blood of rats exposed to hexavalent Chromium. Wistar male rats were used in experiments. All animals were divided into 4 groups. Animals of experimental groups D₂ and D₃ received a 1% solution of food supplement Humilid in a dose of 2 ml/kg of body weight during 28 days. Rats of groups D₁ and D₂ were injected with potassium bichromate (K₂Cr₂O₇) in the dose of 2 mg Cr (VI)/kg of body weight from 14 days of the experiment. Animals of the C group were administered a 0.9% NaCl solution for 14 days. Data were analyzed using the ANOVA test. It has been found that administration of potassium bichromate caused the cytotoxic effect on rat’s organism, inhibition of the synthetic function of the liver, hypoglycemic effect, toxic anemia and leukocytosis. The treatment with humic substances provided a marked normalization of the biochemical and hematological parameters in the blood of chromium-exposed animals. Biologic active supplement Humilid reduces the toxic effect of chromium on the rat body, normalizes metabolism, and exhibits hepatoprotective and adaptogenic effects. Our findings show that Humilid supplementation may be helpful in the abrogation of heavy metal toxicity. Keywords : Humilid; Chromium; rats; hematology; biochemical parameters