Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2020 , Vol 24 , Issue 6
Screening the antimicrobial effect of ferrocene-boronic acid on Pseudomonas aeruginosa using proteomics and metabolomics approach
Engin KOÇAK1,Ceren ÖZKUL2,Gülce TAŞKOR ÖNEL3,Emirhan NEMUTLU4,Sedef KIR4,Meral SAĞIROĞLU2
1Department of Analytical Chemistry, Faculty of Gülhane Pharmacy, Health Sciences University, 06100, Ankara, Turkey
2Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey
3Department of Basic Pharmaceutical Science, Faculty of Pharmacy, Erzincan Binali Yıldırım University, Erzincan, Turkey
4Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey
5Drug and Cosmetic R&D and Quality Control Laboratory (HUNİKAL), Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey
DOI : 10.35333/jrp.2020.240 Antibiotic resistance is one of the most serious global problems around the world. Pseudomonas aeruginosa is gram-negative bacteria and plays important role in local and systemic infections. In our work, we tried to understand mode of antimicrobial action of ferroccene- boronic acid over Pseudomonas aeruginosa via metabolomics and proteomics analysis. In proteomics analysis, we found that ferrocene-boronic acid effects various antimicrobial targets like ATP-dependent DNA helicase RecQ, Transcription-repair-coupling factor and Primasome assembly protein PriA. In metabolomics analysis, the ferrocene-boronic acid induced various metabolites involved in pyrimidine metabolism, lipid and fatty acid metabolism. Moreover, various polyamines like spermine and spermidine, which are very important for antibiotic resistance, pathogenesis and bacterial biofilm formation were decreased by ferroceneboronic acid. We believe that our results will contribute further studies regarding organometallic compounds in microbiology filelds. Keywords : Metabolomics; proteomics; boronic acid; Pseudomonas aeruginosa
Marmara University