Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2019 , Vol 23 , Issue 4
In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract
Mehmet Evren OKUR1,Nihal KARAKAŞ2,3,Ayşe Esra KARADAĞ4,5,Rabia YILMAZ3,Fatih DEMİRCİ6
1University of Health Sciences, Faculty of Pharmacy, Department of Pharmacology, Üsküdar, İstanbul, Turkey
2İstanbul Medipol University, School of Medicine, Department of Medical Biology, Beykoz, İstanbul, Turkey
3İstanbul Medipol University, Regenerative and Restorative Medicine Research Center, Beykoz, Istanbul, Turkey
4İstanbul Medipol University, School of Pharmacy, Department of Pharmacognosy, Beykoz, İstanbul, Turkey
5Anadolu University, Graduate School of Health Sciences, Tepebaşı, Eskişehir, Turkey
6Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, Tepebaşı, Eskişehir, Turkey
DOI : 10.12991/jrp.2019.180 Marrubium vulgare L. (Lamiaceae) is a herbal drug used for centuries for many diseases. In this present study the plant material was acquired from commercial sources in pharma grade (PhEur 8.0) quality. The methanol extract of the aerial parts was evaluated for its in vitro cytotoxic activity by measuring the percentage of viable cells (U87 LN229 and T98G glioblastoma multiforme cell lines) using a luminescence system, and the antioxidant activities by ABTS and DPPH radical scavenging spectrophotometrically. As a result, the methanol extract of M. vulgare showed 48.97 ± 0.82 mg of GA/g corresponding to the total phenolic amounts, and moderate antioxidant activity (1.33 and 2.08) by ABTS• and DPPH• assays. To the best of knowledge, after evaluation of the cytotoxicity on M. vulgare treated U87 (IC50:270.3 μM), LN229 (IC50:343.9 μM) and T98G (336.6 μM) glioblastoma multiforme (GBM) cell lines, significant cytotoxic activities with 69.9% (p= 0.0081) and 71% cell viability (p= 0.0028) was observed in M. vulgare treated U87 and LN229 GBM cell lines, respectively. Overall, further in vitro and in vivo bioactivity studies based on phytochemistry on various M. vulgare preparations are worthwhile in order to discover bioactive secondary metabolites. Keywords : Cytotoxicity-1; glioblastoma multiforme cell lines-2; U87-3; LN229-4; T98G-5; Marrubium vulgare-6
Marmara University