Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Quarterly (January-April-July-October) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2018 , Vol 22 , Issue 1
In vitro antioxidant and cytotoxic activity of Muscari neglectum growing in Turkey
Esra EROĞLU ÖZKAN1,Serpil DEMİRCİ KAYIRAN2,Turgut TAŞKIN3,Mahmoud ABUDAYYAK4
1Istanbul University, Faculty of Pharmacy, Department of Pharmacognosy, 34116, Beyazit, Istanbul, Turkey
2Cukurova University, Faculty of Pharmacy, Department of Pharmaceutical Botany, Balcali, 01330, Adana, Turkey
3Marmara University, Faculty of Pharmacy, Department of Pharmacognosy, 34668, Haydarpasa, Istanbul, Turkey
4Karadeniz Technical University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Trabzon, Turkey
DOI : 10.12991/mpj.2018.43 Muscari species have been used as antirheumatic, stomachic, diuretic, expectorant and anti verruca in folk medicine. The species have also been used as food for humans and animals in Turkey. In this study, the antioxidant and cytotoxic activities of different extracts obtained from Muscari neglectum were studied. The antioxidant capacities of the extracts were evaluated with DPPH free radical scavenging activity, metal chelating capacity and ABTS cation radical scavenging capacity assays. The cytotoxic potentials of the extracts were determined by MTT and LDH assays on HeLa-cells (human cancer cell line) and NRK-52E-cells (rat kidney cell line). The aqueous extracts from M. neglectum bulbs (IC50: 2.83± 0.54 mg/mL) and herba (IC50: 8.52± 1.3 mg/mL) exhibited stronger DPPH free radical scavenging activity than other extracts, respectively. The ethanol extract from M. neglectum bulbs showed the highest ABTS (27.88 %) cation radical scavenging activity. According to the metal chelating activity test results, the aqueous extracts from M. neglectum herba and bulbs showed the strongest metal chelating activity (28.99% and 28.07%, respectively). Keywords : Muscari neglectum, antioxidant activity, cytotoxic activity, Turkey
Marmara University