Editor-in-Chief İlkay Küçükgüzel Associate Editor Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2016 , Vol 20 , Issue 3
Determination of total phenolic and flavonoid content, mineral composition and free radical scavenging activity of Trigonella plicata
Şerife Selma URAS GÜNGÖR1, Sevda GÜZEL1, Ahmet İLÇİM2, Gamze KÖKDİL1
1Mersin Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, 33169, Mersin
2Mustafa Kemal Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Antakya, Hatay
DOI : 10.12991/mpj.20162084302 The aim of the present study was to determine total phenolic and flavonoid content and free radical scavenging activity and mineral composition of Trigonella plicata Boiss. & Bal. from Turkey. Total phenol, flavonoid content and free radical scavenging activity of the aqueous methanolic extract obtained from seeds was measured spectrophotometrically by Folin-Ciocalteu, the AlCl3 and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The phenolic content was 64.11±0.36 mg gallic acid equivalent (GAE)/g dry weight while the flavonoid content was 51.02±0.056 mg rutin equivalent (RE)/g dry weight. In free radical scavenging assay, inhibition % of the extract (1g/L)was found to be 50.1±0.039. The mineral composition of the seeds were determined by ICP-MS and potassium was main mineral in the seeds. The other main elements, in descending order by quantity, were Ca, P, Mg and Na. Keywords : T. plicata, toplam phenol and flavonoid, radical scavenging activity, mineral composition
Marmara University