Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2015 , Vol 19 , Issue 1
Antioxidant, antimicrobial activities and total flavonoid contents of Cirsium bulgaricum DC. leaf extracts
Ayca KARASAKAL1, A.Sukru DEMIRCI2, Nazan TOKATLI DEMIROK3, Evren CABI4
1Department of Chemistry, Art and Science Faculty, Namik Kemal University, 59100 Tekirdag - TURKEY
2Department of Food Engineering, Agriculture Faculty, Namik Kemal University, 59100 Tekirdag – TURKEY
3Department of Nutrition and Dietetics, School of Health, Namik Kemal University, 59100 Tekirdag – TURKEY
4Department of Biology, Art and Science Faculty, Namik Kemal University, 59100 Tekirdag – TURKEY
DOI : 10.12991/mpj.2015198609 In this study, the antioxidant activities of methanol and acetone extracts of Cirsium bulgaricum DC. (Asteraceae) were evaluated by five antioxidant assays, including phosphomolybdate method, cupric ion reducing capacity (CUPRAC), 2,2"-azinobis(3-ethylbenzothiazoline-6- sulfonicacid)/persulfate) ABTS/Persulfate, N, N-dimethyl-pphenylenediamine (DMPD) and a Ce(IV)-based reducing capacity (CERAC) methods. The 80% methanol and acetone extracts showed 0.005-6.16±0.03 mmol/g and 0.004- 5.02±0.02 [(trolox (TR), catechin (CT), epicatechin (EC), rutin (RT), quercetin (QR), naringin (NG) in CUPRAC and ABTS/Persulfate methods, respectively]. According to DMPD methods, inhibition effect were showed 2.3%±0.02 for 80% methanol and 4.6%±0.07 for acetone. Ascorbic acid equivalent mmol (AA)/g extract in CERAC method was found to be 0.0834±0.09 for 80% methanol and 0.5622±0.05 for acetone. Ascorbic acid equivalent μg ascorbic acid (AA) /mL extract were found 807.6±0.07 for 80% methanol and 292.4±0.01 for acetone in phpsphomolybdate method. In the addition to the antioxidant activity of the extract, the total flavonoid content was measured. Total flavonoid content of 80% methanol and acetone extracts was found to be 105.5-645±0.04 μg/ml quercetin and rutin equivalent. For the determination of antibacterial activities Escherichia coli (E.coli) ATCC 25922, Escherichia coli(E.coli) O157:H7 ATCC 33150, Salmonella E n t e r i t i d i s(S.E n t e r i t i d i s ) AT C C 1 3 0 7 6 , Listeria monocytogenes(L.monocytogenes) AT C C 7 6 4 4 , Staphylococcus aureus(S.aureus) ATCC 25923 bacteria were used as test bacteria. Acetone extracts showed antibacterial activity against all of the tested bacteria ranged between 17.00±1.72- 19.88±1.75 mm and 80% methanol extracts showed antibacterial activity against all of the tested bacteria ranged between 11.35±1.43-14.43±1.32 mm. Keywords : Antioxidant Activity, Antimicrobial Activity, Total Flavonoid Contents, Cirsium bulgaricum
Marmara University