Editor-in-Chief İlkay Küçükgüzel Associate Editor Aslı Türe Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2014 , Vol 18 , Issue 3
In Vitro Investigation of The Effects of Some Antibiotics on Polymorphonuclear Leukocyte Functions and Oxidative Stress in Hemodialysis Patients
Refiye GARIP1, Umran SOYOGUL GURER1, Burcak GURBUZ1, Esra DALKILIC1, Sukru PALANDUZ2, Fatma Nilgun AYSUNA3, Azize YAMAN SENER4
1Marmara Üniversitesi Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
2İstanbul Üniversitesi Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Tıbbi Genetik Bilim Dalı, İstanbul, Türkiye
3İstanbul Üniversitesi Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Nefroloji Bilim Dalı, İstanbul, Türkiye
4Marmara Üniversitesi Eczacılık Fakültesi, Biyokimya Anabilim Dalı, İstanbul, Türkiye
DOI : 10.12991/mpj.2014187241 In our study we were investigated the in vitro effects of therapeutic concentrations of vancomycin (40 μg/ml), daptomycin (60 μg/ml), linezolid (19 μg/ml), levofloxacin (7 μg/ml), fusidic acid (36 μg/ml) and meropenem (24 μg/ml) o n t h e m a l o n d i a l d e h y d e ( M D A ) l e v e l s a n d polymorphonuclear leukocyte (PMN) functions (phagocytic and intracellular killing activity) in hemodialysis patients. PMNs were isolated from venous blood by ficoll-hypaque gradient centrifugation method. Phagocytic activity and intracellular killing activity were assayed by modified Alexander"s method. MDA level was detected by Beuge method which is as an indicator of lipid peroxidation. All results was evaluated by statisticaly. Vancomycin, daptomycin, linezolid, levofloxacin, fusidic acid and meropenem at therapeutic concentrations did not affect both PMN functions of HD patients before and after HD (p>0.05). It was observed that vancomycin, levofloxacin, fusidic acid and meropenem at therapeutic concentrations increased PMN"s MDA levels of HD patients before HD but decreased MDA levels after HD (p<0.05). Daptomycin and linezolid did not change PMN"s MDA levels of HD patients before and after HD (p>0.05). Keywords : Hemodialysis, oxidative stress, polymorphonuclear leukocyte functions
Marmara University