¹Department of Pharmacology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey DOI : 10.29228/jrp.421 Chronic wounds in diabetes are common and serious health problems which also create significant economic burden in society. A comprehensive treatment has not yet been found in chronic wounds and new treatment recommendations are needed. The beneficial effects of metformin on wound healing was shown previously in healthy or diabetic animal models. Hyaluronic acid was also found to accelerate wound closure in clinical trials as well as animal models. Metformin and hyaluronic acid have been shown to be beneficial for wound healing in diabetes in in vivo studies, but their effect on wound healing under in vitro diabetic conditions have not yet been enlightened. In this study; we investigated the effects of metformin and hyaluronic acid on wound healing in mouse fibroblast cells under high glucose conditions. We used the 2,3-bis-(2-methoxy-4-nitro-5- sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and scratch migration assays to examine fibroblast cell viability and migration in wound healing respectively. We showed that metformin and hyaluronic acid enhanced wound healing in fibroblasts incubated with high glucose by increasing cell viability, proliferation and migration. This is the first study to examine the effect of metformin, hyaluronic acid, or their combination on wound healing under high glucose conditions in vitro. Keywords : Fibroblast; hyaluronic acid; metformin; wound healing; diabetes