Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 1
Development and radiolabeling of lipid nanoparticles with [99mTc]Tc-HMPAO: Characterization, stability, cytotoxicity and cell binding studies
1Department of Radiopharmacy, Faculty of Pharmacy, Ege University, Bornova 35100 Izmir, Turkey DOI : 10.29228/jrp.106 In this study, we aimed to develop new lipid based nanoparticles (LPNs) and radiolabeled LPNs with [99mTc]Tc-HMPAO to investigate its cell binding capacity comparatively with [sup>99mTc]Tc-HMPAO on different cancer cells. According to obtained results, LPNs with zeta potential of -27.4 ± 0.95 mV, particle size of 93.5 ± 1.17 nm, and polydispersity index of 0.35 ± 0.04 were successfully developed. The optimum radiolabeling efficiency was found to be above 90% at 15-min of incubation time. The cell binding capacity of [99mTc]Tc-HMPAO-LPNs was found to be higher than [99mTc]Tc-HMPAO in cancer cell lines. The results demonstrated that [99mTc]Tc-HMPAO-LPNs may be a promising agent for cancer diagnosis alternatively to [99mTc]Tc-HMPAO. Keywords : Lipid nanoparticles; radiopharmaceuticals; HMPAO; cell binding; cancer diagnosis
Marmara University