Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2021 , Vol 25 , Issue 3
Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LC-ESI-MS/MS with chemometric approach
1Université des Frères Mentouri-Constantine 1, Laboratoire d’Obtention de Substances Thérapeutiques (LOST), 25000 Constantine, Algeria
2University Badji-Mokhtar Annaba, Faculty of Medicine, 23000 Annaba, Algeria
3Department of Chemistry, Faculty of Sciences, Mugla Sıtkı Koçman University, 48121 Muğla, Turkey
4Köyceğiz Vocational School, Mugla Sıtkı Koçman University, 48121 Muğla, Turkey
5Department of Pharmacognosy, Faculty of Pharmacy, Dicle University, 21280 Diyarbakır, Turkey
DOI : 10.29228/jrp.13 The fractions (Fr.1-5) of the hydromethanolic extract of Abies numidica cones were studied for their polyphenol constituents, antioxidant, and anticholinesterase activities. The β-carotene-linoleic acid, cupric reducing power (CUPRAC), DPPH scavenging, and ABTS radical scavenging assays were used to determine antioxidant activity. Fr.3 exhibited the highest antioxidant activity in ABTS test (IC50: 1.29 μg/mL), b-carotene-linoleic acid test (IC50: 18.6 μg/mL) and CUPRAC (A0.5: 33.8 μg/mL) assays. Three fractions (Fr.2-4) promising antioxidant activity were analyzed using LC-MS/MS for their phenolic compositions. Taxifolin (155.9-2816.2 μg analyte/g extract), hyperoside (353.0-2045.5 μg/g), vanillin (1488.9-1529.9 μg/g), tannic acid (1281.6-1416.8 μg/g), rosmarinic acid (1063.1-1149.3 μg/g), coumarin (683.0-772.6 μg/g), quercetin (881.0 μg/g), and catechin (277.7-400.2 μg/g) were identified in the antioxidant fractions. Moreover, the anticholinesterase activity was also performed using the in vitro spectroscopic Ellman method. The antioxidant fractions exhibited moderate butyrylcholinesterase inhibitory activity. The multi-ware analysis was performed to understand the origin of bioactivities. According to principal component analysis, it is detected that the hyperoside, catechin, taxifolin, and tannic acid were responsible for the antioxidant activity. Therefore, the cones of Abies numidica can be considered as potent antioxidant and anticholinesterase sources in food and pharmaceutical industries, commercially. Keywords : Abies numidica; LC-MS/MS; antioxidant activity; anticholinesterase activity; taxifolin; hyperoside
Marmara University