Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 1989 , Vol 5 , Issue 2
Isolation of the point mutation of the enoyl reductase-minus mutant (FAS I-246), in Saccharomyces cerevisae
Turgut Ulutin
The nuckaotide sequence of the fas I gene specifically coding the enoyl reductase domain has been shown to be cloned into the plasmid Yep FAS I. The studies on the enoyl reductase minus-mutant (fas I-246) involved a yeast transformation technique using cloned plasmids, with yeast DNA fragments cleaved from the YEpFAS I clone. By cloning various overlapping yeast DNA fragments of YEpFAS I it was hoped to isolate the point mutation to a suitably fragment, which could be sequenced and the mechanism behind the mutation elucidated. Keywords :
Marmara University