Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2024 , Vol 28 , Issue 3
Therapeutic effect of Anethum graveolens L. (Dill) seed extract for the treatment of dextran sodium sulfate (DSS) induced ulcerative colitis in Wistar rats
1Department of Pharmacology, Student of Pharmacy, Madras Medical College, Chennai, India
2Department of Pharmacology, Faculty of Pharmacy, Madras Medical College, Chennai, India
DOI : 10.29228/jrp.738 The purpose of this study was to determine whether the Anethum graveolens L. seed (AGS) extract has any anti-oxidant, anti-inflammatory, or immune-modulatory activities against Ulcerative colitis (UC). AGS extract has found widespread use in folk medicine. Twenty seven (n=27) adult female Wistar albino rats were employed for the experiment involving Dextran Sodium Sulfate (DSS) induced UC. These rats were distributed randomly into five groups, each consisting of six animals (n=6), with the exception of group 1, which had three animals (n=3). Group 1 received 0.5% Carboxy methyl cellulose (CMC) (p.o.), Group 2 received 5% DSS (p.o.), Group 3 received 350 mg/kg b.w. mesalazine (p.o.), and Groups 4, 5, respectively, received 200 and 400 mg/kg AGS extract (p.o). Along with administering DSS, the treatment lasted for 12 days. In comparison to the DSS-treated group, groups 4, and 5, there was a considerable weight gain. Comparing groups 4 and 5 to group 2, the groups 4 and 5 showed a substantial decrease in Disease Activity Index (DAI), colon damage (p<0.05), spleen weight (p<0.05), serum IL-6 (p<0.0001), TNF-α (p<0.01), and IL-17 (p<0.01) levels. Rats in groups 4 and 5 had significantly higher blood IL-10 levels (p<0.05) and longer colons than rats in group 2 (p<0.05). The good results, which showed an improvement in the state of the colon epithelium after AGS extract in treatment groups, were validated by a histopathological study. In summary, AGS extract may be able to control inflammatory reactions and safeguard the colon epithelium in UC. These results support the possible therapeutic application of AGS extract. Keywords : Anethum graveolens L.; ulcerative colitis; dextran sodium sulfate; antioxidant; anti-inflammatory
Marmara University