¹Faculty of Pharmaceutical Sciences, Burapha University, Chonburi 20131, Thailand
²Pharmaceutical Innovations of Natural Products Unit (PhInNat), Burapha University, Chonburi 20131, Thailand DOI : 10.29228/jrp.535 Buchanania lanzan Spreng and Buchanania siamensis Miq. (Anacardiaceae) are traditional medicinal plants that are rich in phenolic compounds and are used for the treatment of inflammatory diseases and pain. In the present study, the antioxidant potential of the distilled water and 80% ethanol extracts of B. lanzan and B. siamensis leaves was evaluated by various antioxidant assays, including DPPH, ABTS, FRAP, and ROS assays. The simultaneous HPLC determination of three major compounds was developed and validated. In addition, the total phenolic contents of each extract were also measured. From HPLC parameter validation, good linearity was achieved, with a correlation coefficient greater than 0.997. The method provided acceptable accuracy, specificity, and precision, suggesting that it could be suitably used for the quantification of the major compounds (myricitrin, isoquercitrin, and quercitrin) in the crude extracts of B. lanzan and B. siamensis. The results of the antioxidant assays showed that the ethanol extracts exhibited higher antioxidant activity than the aqueous extracts. The ethanol extract of B. lanzan leaves expressed the most antioxidant activity and could serve as a potential antioxidant source. Moreover, our validated technique would be beneficial for the quality control of the extract. Keywords : Antioxidant; Buchanania; HPLC; ROS; validated