Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 6
Evaluation of antidiabetic potential and protective effects of Acioa barteri against biochemical changes in alloxaninduced diabetic rats
Robert Ikechukwu UROKO1,Chinedu AGUWAMBA2,Eberechi Lolly MBANASO3,Benedict Chidozie UMEZURIKE4,Elisha UKO OGWO5,Chinonso Friday AARON6,Paul Chukweumaka NWEJE-ANYALOWU2,Mercylyn Ezinne UCHE7
1Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B. 7267, Umuahia, Abia State, Nigeria
2Biochemistry Unit, Department of Chemical Sciences, Faculty of Sciences, Clifford University, Owerrinta, Abia State, Nigeria
3Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine and Health Sciences, Abia State University, Uturu, Nigeria
4Department of Public Health, Gregory University Uturu, Abia State, Nigeria
5Department of Human Physiology, College of Medicine and Health Sciences, Abia State University, Uturu, Nigeria
6Department of Biochemistry, Faculty of Biological and Physical Sciences, Abia State University, Uturu, Nigeria
7Department of Medical Biochemistry, College of Medicine, Imo State University
DOI : 10.29228/jrp.518 Acioa barteri is a medicinal plant commonly known as Monkey fruit that grows in the tropical rain forests in many countries in West Africa. The plant extracts are useful in traditional medicine against diverse diseases and medical conditions. This study evaluated the antidiabetic and protective effect of Acioa barteri extract (EEABL) against biochemical changes in alloxan-induced diabetic rats. The lethal dose of EEABL was evaluated using standard procedure. Antidiabetic and protective properties of EEABL were evaluated using sixty mature male albino rats selected into six groups containing ten rats each. Group 1 was the normal control rats without alloxan-induction but received 2mL/kg of normal saline. Groups 2 – 6 were rats induced diabetes by the intraperitoneal administration of 150 mg/kg alloxan-monohydrate dissolved in normal saline. Group 2 was the diabetic control, and group 3 was treated with 3 mg/kg Glibenclamide. Groups 4, 5, and 6 were treated with 200, 400, and 800 mg/kg EEABL, respectively for 28 days. The results of the acute toxicity study of EEABL showed that it has a lethal dose (LD50) value above 5000 mg/kg. The phytochemical study showed that EEABL contains alkaloids (96.89±0.42 mg/100g), flavonoids (105.98±1.73 mg/kg), and phenols (77.58±0.29 mg/kg) in high concentrations. While tannins (13.13±0.43 mg/100g) and terpenes (18.24±0.78 mg/100g) were detected in moderate concentration in EEABL, cardiac glycoside (10.39±0.20 mg/100g), steroids (8.28±0.19 mg/100g) and saponins (6.34±0.19 mg/100g) were found to be present in low concentrations. Treatment with EEABL lowered blood glucose, urea, and creatinine significantly in the alloxan-induced diabetic rats compared to the diabetic control. Treatment with EEABL also improved haematological parameters, antioxidant vitamins (B12, B6, B2, C, and E), serum electrolytes, and lipid profile in the alloxan-induced diabetic rats compared to the diabetic control. This study revealed that EEABL has antidiabetic effects and confers protection against biochemical changes in alloxaninduced diabetic rats. Keywords : Acioa barteri; antioxidant vitamins; hyperglycaemia; lipid profile; phytochemicals; renal functions
Marmara University