Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2023 , Vol 27 , Issue 2
Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats
Serdal KURT1,Ugur SEKER2,Murat Onur YAZLIK3,Engin ER4,Muhammed Mesud HÜRKUL5,Sevda SOKER6
1Department of Veterinary, Elbistan Vocational School, Kahramanmaras Istiklal University, Kahramanmaras, Türkiye
2Department of Histology and Embryology, Faculty of Medicine, Harran University, Sanliurfa, Türkiye
3Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Ankara University, Ankara, Türkiye
4Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Türkiye
5Department of Pharmaceutical Botany, Faculty of Pharmacy, Ankara University, Tandoğan, Ankara, Türkiye
6Department of Histology and Embryology, Faculty of Medicine, Dicle University, Diyarbakir, Türkiye
DOI : 10.29228/jrp.348 The study investigated the major phenolic compounds of Aloe vera leaf (AVL) by the high-performance liquid chromatography (HPLC) method, and its protective effect on ovaries under oxidative stress of streptozotocininduced diabetic rats. The study was conducted on control (untreated healthy rats; C), diabetes (untreated diabetic rats; D) and diabetes+A. vera treatment (diabetic rats treated with A. vera; D+A) groups. D+A group was given an ethanolic extract of A. vera (300 mg/kg) for 14 days. Major phenolic compounds of AVL were chlorogenic acid and rutin. alanine transaminase (ALT) and aspartate aminotransferase (AST) levels increased in D group compared to others groups (P<0.01). Alkaline phosphatase (ALP) and albumin levels were lower and higher in the C group than in other groups, respectively (P<0.01). In the D group compared to other groups, oxidative stress index (OSI) and total oxidant status levels increased, while total antioxidant status level decreased in both ovarian tissues and blood (P<0.01). Preantral and antral follicle counts decreased in the D group according to other groups (P<0.01) and atretic follicle count increased (P<0.05). Proliferating cell nuclear antigen (PCNA) expression level (P<0.01), and B-cell lymphoma-2-associated-Xprotein (Bax; P<0.01) and tumor necrosis factor-alpha (TNF-α) expression levels (P<0.05) decreased and increased in group D according to other groups, respectively. In conclusion, treatment with AVL decreased OSI, improved follicular dynamics, and restored Bax, TNF-α and PCNA expression in diabetic rats. Keywords : Aloe vera; antioxidant; diabetes; HPLC; ovarian; rat
Marmara University