Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 3
Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method
HAJER ASWAISSI1,EBRU TÜRKÖZ ACAR2
1Yeditepe University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Istanbul, Turkey
2Yeditepe University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Istanbul, Turkey
DOI : 10.29228/jrp.163 In this study, a new high performance liquid chromatography (HPLC) analysis method was developed for the simultaneous determination of flurbiprofen (F) and thiocolchicoside (T) in pharmaceutical products. A C18 column (Agilent Poroshell 120 EC-C18) was used as the stationary phase. Elution of the analytes was achieved by using gradient elution system. Mobile phase A was an acetate buffer (100 mM, pH 4.8) and mobile phase B was acetonitrile. The flow rate for the method developed was 0.5 ml/min. The temperature of the column compartment was set at 40°C and the injection volume was 5 μl. Monitoring of the analytes was carried out at 248 nm. The method was found linear in the concentration range between 5-100 ppm. The correlation coefficient value was calculated as 0.999 for F and 0.999 for T. The method was found suitable in terms of accuracy, precision, specificity, linearity, and robustness ruggedness. Furthermore, it was applied successfully for the analysis of commercial tablets and gel samples that contains F and T without any time-consuming pre-procedure. The recovery values for Fand T were found as 99.5% and 97.0% in tablet formulations and 98.6% and 99.3% in gel formulations, respectively. All results were acceptable and this confirmed that the method is suitable for its intended use in routine quality control and an assay of drugs. Keywords : Flurbiprofen, Thiocolchicosoide, HPLC, analysis, validation, commercial dosage forms
Marmara University