Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 3
An In-Silico Approach for Analysing the interplay of Hepatitis B viral X protein with Human Adaptin protein
Prachie SHARMA1,Kapila KUMAR2,Kamal RAWAL3
1Department of BIOECHNOLOGY, Faculty of ENGINEERING AND TECHNOLOGY, MANAV RACHNA INTERNATIONAL INSTITUTE OF RESEARCH AND STUDIES, Faridabad, Haryana, India
2Department of BIOECHNOLOGY, Faculty of ENGINEERING AND TECHNOLOGY, MANAV RACHNA INTERNATIONAL INSTITUTE OF RESEARCH AND STUDIES, Faridabad, Haryana, India
3AMITY INSTITUTE OF BIOTECHNOLOGY, Amity University, Noida, Uttar Pradesh, India
DOI : 10.29228/jrp.145 Hepatitis B virus (HBV) is the predominant cause for the liver-related malignancies worldwide. Recently, clathrin mediated pathway has been found to assist the Hepatitis B virion in entering the host cell successfully. The role of the viral L protein subunit (LHB) has been understood to interact with clathrin proteins during the clathrin mediated endocytosis. The role of the viral X protein is necessitated here owing to the regulatory nature of this protein during the pathogenesis, which however has been quite elusive. Therefore, an in-silico study has been designed to assess the interaction possibilities between the adaptor protein Gamma 2 adaptin and the regulatory viral protein X (HBX). This study is designed to predict the interaction between Viral HBx protein and human Gamma 2 adaptin protein of clathrin mediated endocytic pathway during the Hepatitis B viral infection using insilico protein protein docking tools. The protein complexes Gamma-2-Adaptin-HBx and Gamma-2-Adaptin-LHB were then docked using Cluspro, Hex and HDock respectively. We found that Gamma-2-Adaptin-HBx binding energies using Cluspro and hex we more negative as compared to the ones obtained for the Gamma-2-Adaptin-LHB complex. This study indicates towards the HBX viral protein in being on of the important interactors of Gamma 2 adaptin in addition to LHB. Keywords : Hepatitis B virus; HBV; Viral Protein; Clatherin; Endocytosis; Human
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