Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2022 , Vol 26 , Issue 1
Protective effect of Enhydra fluctuans DC. aerial against insulitis in alloxan-induced diabetic rats
Rina DELFITA1,Dahelmi DAHELMI2,Djong Hon TJONG2,Suhatri SUHATRI3
1Department of Biology Education, Faculty of Tarbiyah and Teacher Training, Institut Agama Islam Negeri (IAIN) Batusangkar, West Sumatra, Indonesia
2Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, West Sumatra, Indonesia
3Department of Pharmacy, Andalas University, West Sumatra, Indonesia
DOI : 10.29228/jrp.115 This study aimed to assess the protective effect of the n-hexane fraction of Enhydra fluctuans aerial against insulitis in alloxan-induced diabetic rats. A total of 30 male rats (five normal rats and 25 diabetic rats) were assigned to one of six groups (n = 5/groups): group 1 is the normal rat group (GN) was without treatment, group 2 is diabetic rat was given 0.5% Na-CMC (G0), group 3 is diabetic rat was given glibenclamide 0.45 mg/kg body weight (G1), group 4, 5, and 6 are diabetic rats were given n-hexane fraction doses of 57.03, 114.06, and 171.09 mg/kg body weight respectively. The experiment was carried out over 21 days. Blood glucose was measured on the first and latest day of treatment. Furthermore, insulin levels, the number of pancreatic β-cell, and degrees of insulitis were evaluated. The n-hexane fraction reduced blood glucose, increased insulin levels, and increased the number of pancreatic β-cell significantly in alloxan-induced diabetic rats. The administration of the n-hexane fraction at a dose of 57.03 mg/kg body weight exerted the best protective effect against insulitis and promoted regeneration of the islet of Langerhans. The results of this study indicate that this herb could effectively reduce insulitis and promote the regeneration of pancreatic tissue under diabetes. Thus, E. fluctuans has the potential to be developed as a novel diabetes drug. Keywords : Alloxan; antidiabetes; Enhydra fluctuans; hyperglycemia; insulitis
Marmara University