Editor-in-Chief
Hatice Kübra Elçioğlu
Vice Editors
Levent Kabasakal
Esra Tatar
Online ISSN
2630-6344
Publisher
Marmara University
Frequency
Bimonthly (Six issues / year)
Abbreviation
J.Res.Pharm.
Former Name
Marmara Pharmaceutical Journal
Journal of Research in Pharmacy
2021 , Vol 25 , Issue 4
Volatiles and fatty acid analyzes of Tripleurospermum decipiens (Fisch & C. A. Mey) Bornm and investigation of the extracts for antimicrobial and enzyme inhibitory activities
1Department of Pharmacognosy, Faculty of Pharmacy, Trakya University, Edirne, 22030 Turkey2Faculty of Pharmacy, Trakya University, Edirne, 22030 Turkey
3Medicinal Plant, Drug and Scientific Research Center (AUBIBAM), Anadolu University, Eskişehir, 26470, Turkey
4Department of Pharmaceutical Botany, Faculty of Pharmacy, Anadolu University, Eskişehir, 26470 Turkey
5Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskişehir, 26470, Turkey DOI : 10.29228/jrp.34 In this study, the volatiles were obtained from aerial parts of Tripleurospermum decipiens (Fisch & C.A.Mey) Bornm with microsteam distillation - solid phase microextraction (MSD-SMPE) technique. The fatty acids from T. decipiens were obtained by using the microextraction method with subsequent transesterification with boron trifluoride reagent. The chemical compositions of the volatiles and fatty acid methyl esters was analyzed by Gas Chromatography (GC) and Gas Chromatography/ Mass Spectrometry (GC / MS) techniques. n-Hexane (TDH) and methanol (TDM) extracts of T. decipiens were investigated for acetylcholineterase (AChE), -amylase enzyme inhibitions and antimicrobial activities. In vitro inhibition of AChE was monitored by using Ellman’s chromogenic agent, and antidiabetic effects of the extracts were spectrophometrically evaluated by inhibition of porcine pancreatic -amylase enzyme. Antimicrobial activities of the extracts were evaluated against Salmonella enterica ATCC 14028, Bacillus subtilis subsp spizizeni ATCC 6633, B. subtilis ATCC 19654, Staphylococcus aureus ATCC 6538, Klebsiella aerogenes ATCC 13048, Candida parapsilosis ATCC 22019 by microdilution method. In vitro -amylase enzyme inhibitory activity was observed for methanol extract (55.6%) at 10 mg/mL. The highest AChE inhibition was recorded for n-hexane extract (58.8%) at concentration (10 mg/mL). The extracts were found to be effective against B. subtilis ATCC 19654, B. subtilis subsp spizizeni ATCC 6633, S. aureus ATCC 6538 and C. parapsilosis ATCC 22019. Keywords : Tripleurospermum decipiens; essential oil; extract; acetylcholineterase; &alpha-amylase; antimicrobial