Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Journal of Research in Pharmacy 2021 , Vol 25 , Issue 3
Effect of food supplement of humic origin on the hematological and biochemical parameters in the Cr (VI) exposed rats
Oksana BUCHKO1,Viktoriia HAVRYLIAK2,Andriy PYLYPETS3,Taras BUCHKO4
1National Reference Control Laboratory of Veterinary Drug Residues in product of animal origin and food additives, State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, Lviv, Ukraine
2Department of Technology of Biologically Active Substances, Pharmacy and Biotechnology, Lviv Polytechnic National University, Lviv, Ukraine
3Laboratory of biochemistry adaptation and ontogenesis of animals, Institute of Animal Biology, Lviv, Ukraine
4Faculty of Biology, Ivan Franko National University of Lviv, Ukraine
DOI : 10.29228/jrp.17 The study aimed to investigate the effect of Humilid, a biologically active food supplement, on biochemical and hematologic parameters in the blood of rats exposed to hexavalent Chromium. Wistar male rats were used in experiments. All animals were divided into 4 groups. Animals of experimental groups D2 and D3 received a 1% solution of food supplement Humilid in a dose of 2 ml/kg of body weight during 28 days. Rats of groups D1 and D2 were injected with potassium bichromate (K2Cr2O7) in the dose of 2 mg Cr (VI)/kg of body weight from 14 days of the experiment. Animals of the C group were administered a 0.9% NaCl solution for 14 days. Data were analyzed using the ANOVA test. It has been found that administration of potassium bichromate caused the cytotoxic effect on rat’s organism, inhibition of the synthetic function of the liver, hypoglycemic effect, toxic anemia and leukocytosis. The treatment with humic substances provided a marked normalization of the biochemical and hematological parameters in the blood of chromium-exposed animals. Biologic active supplement Humilid reduces the toxic effect of chromium on the rat body, normalizes metabolism, and exhibits hepatoprotective and adaptogenic effects. Our findings show that Humilid supplementation may be helpful in the abrogation of heavy metal toxicity. Keywords : Humilid; Chromium; rats; hematology; biochemical parameters
Marmara University