¹Department of Pharmaceutical Botany, Faculty of Pharmacy, Atatürk University, Erzurum, 25240, Turkey
²Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, 61080, Turkey
³Department of Chemistry, Faculty of Sciences, Atatürk University, Erzurum, 25240, Turkey
⁴Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, 61080, Turkey
⁵Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, Trabzon, 61080, Turkey DOI : 10.29228/jrp.6 The roots and pseudofruits of Rosa pimpinellifolia (Rosaceae) are used in traditional medicine as hemorrhoids, various infections, abdominal pain complaints, heart disease, flu, cold, and anemia treatment in Turkey. In this study, the phytochemical composition of the R. pimpinellifolia was revealed by using chromatographic and spectroscopic techniques. Two condensed tannin precursor, (-)-catechin (1), the mixture of catechin and epicatechin (2) and three triterpenoid glycosides, kaji-ichigoside F1 (3), 2α,3β,19α trihydroxyurs-12-en-28-O-β-D-glucopyranoside (4), 2α,3β,19α,23β-tetrahydroxyurs-12-en-28-O-β-D-glucopyranoside (5) were isolated from the roots of R. pimpinellifolia. The isolation studies were performed by column chromatography. The chemical structures of the isolated compounds were elucidated with 1H and 13C NMR spectroscopy. Total phenolic compound contents were determined as 31.1937 mg/g dry extract in the pseudofruit; 9.8909 mg/g dry extract in the root via HPLC. The seeds of R. pimpinellifolia were extracted with diethyl ether in Soxhlet apparatus. The methyl esters of fatty acid were obtained from fixed oil and analyzed by GC/MS. The total ratio of unsaturated fatty acids in the seeds was 93.4%; containing 51.40% ratio of linoleic acid and 41.20% ratio of homo-γ-linolenic acid. Keywords : Rosa pimpinellifolia; Triterpenoid glycosides; phenolic compounds; fatty acid