The thin layer chromatographic analysis of some basic drugs and the investigation of the influence of the biological matrix on the analysis
Ömer Ersoy,R. Sema Aydemir
In thi study, the analysis of some basic drugs was performed by means of thin layer chromatographic. By choosing a suitable solvent system, the reproducibility of the results was investigated and it was tried to standardise the method in our laboratory conditions. Thin layer chromatographic studies were performed with 11 basic drug substances both on the plates prepared in our laboratory using silicagel 60 HF254 and on the commercial chromatographic plates covered with the same adsorbent. The solvent system was Methanol: Ammonia, %25 (100:1.5). In addition to the chromatography of the pure drug substances, urine samples spiked with these basic drugs were also after solvent extraction chromatographed and the influence of the biological matrix on the analysis was investigated.
In several trials, the averages of the Rfx100 values and their standard deviations for the pure drugs and for the same substances but with the fractions after the solvent extraction from spiked urine samples were achieved, and the values obtained in the two types of chromatographic plates were compared. The differences between the averages of the Rfx100 values of the pure basic drugs and those extracted from urine were found statistically significant for the 7 of the 11 substances on the chromatographic plates prepared in our laboratory and for the 5 on the commercial chromatographic plates. Therefore, it can be said that the biological matrix affects the thin layer chromatographic behaviour of these drugs in urine.
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