¹Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, Ankara, Turkey
²Department of Pharmaceutical Botany, Faculty of Pharmacy, Ankara University, Ankara, Turkey DOI : 10.35333/jrp.2020.249 In traditional medicine, Lamiaceae plants are widely used as a cure for various health problems. Nepeta humilis is one of the Lamiaceae plants of Iranian-Turkish district growing naturally in the South East Anatolia. The present study was designed to investigate the radical scavenging activity and total phenolic content of Nepeta humilis as well as to determine the phenolic constituents of the extracts of flowers, leaves and roots using a previously validated RP-HPLC-DAD method for the first time. The total phenolic contents of flowers, leaves and roots were found as 123.18 ± 1.01, 66.20 ± 0.49 and 54.77 ± 1.23 mg GAE/g extract, respectively. In accordance with the higher total phenolic content, flower extract of the plant displayed more radical scavenging activity with both assays. HPLC results revealed that rosmarinic acid was detected as one of the main compounds in all the investigated parts of the plant, especially in the flowers (0.397 ± 0.01 g/100g dry weight). Also, chlorogenic acid, luteolin and apigenin were found in particular amount in the flower extract. Nepeta humilis is rich in phenolic compounds and can be evaluated as a promising antioxidant source. Keywords : Nepeta; Lamiaceae; radical scavenging; HPLC; phenolic compounds