¹PG and Research Department of Botany, Government Arts College, Coimbatore - 641018, Tamilnadu, India DOI : 10.35333/jrp.2020.247 Durian (Durio zibethinus Murr.) is a seasonal tropical fruit of Southeast Asia. It is a good source of carbohydrates, proteins, fibres, vitamins B and C. The fruit extract was prepared using the mixture of Ethanol and 0.2 M HCl (1:1, v/v). Phytochemical, antioxidant and anticancer properties of durian were investigated in this study. Phytochemical analysis exhibited the highest total phenolics value of 116.55±1.51 mg GAE/g extract and the highest total flavonoid value of 92.37±9.27 mg RE/g extract. In antioxidant studies, ABTS and NO assays of durian fruit extracts showed a greater value in antioxidant activity against control. Whereas, the results derived from DPPH, FRAP and other assays showed lower antioxidant potential compared to the standard. Since durian fruit possess rich phenolic content, which is the principal factor of the antioxidant activity, it shows excellent antioxidant potentials against various assay methods. MTT assay was used for the cytotoxicity of durian extract against Chang Liver cell lines. The percentage of cytotoxicity increases with the higher concentrations of durian extract. The maximum cytotoxicity (74%) of fruit extract was recorded at 100 μg/ml of the fruit extract. It shows durian fruit extract to have an excellent anti-proliferative and anticancer activities against Chang liver cell lines. Hence durian fruit can be utilized as a potential source for rich bioactive compounds with nutraceutical and pharmaceutical applications. Keywords : Durian; Durio zibethinus; antioxidants; anticancer; phytochemical