The nuckaotide sequence of the fas I gene specifically coding the enoyl reductase domain has been shown to be cloned into the plasmid Yep FAS I. The studies on the enoyl reductase minus-mutant (fas I-246) involved a yeast transformation technique using cloned plasmids, with yeast DNA fragments cleaved from the YEpFAS I clone. By cloning various overlapping yeast DNA fragments of YEpFAS I it was hoped to isolate the point mutation to a suitably fragment, which could be sequenced and the mechanism behind the mutation elucidated. Keywords :