¹Department of Biotechnology and Bioinformatics, Jnanasahyadri, Kuvempu University, Shankaraghatta – 577 451, Shimoga Dist. Karnataka, India DOI : 10.12991/jrp.2018.114 Thevetia peruviana (Pers.) K. Schum, well known as Karvira in the Ayurvedic text. The plant has been traditionally used for the treatment of wounds, inflammation and diverse types of skin disorders. The present study was designed to analyze the antioxidant and anti-inflammatory mediated wound healing potential of T. peruviana leaf (TPLE) and fruit rind (TPFE) ethanolic extracts. To establish the efficacy, the study was designed using in vitro antioxidant activities, carrageenan-induced inflammation model and incision, excision, dead space wound models associated with antioxidant enzymes, tissue biochemical and inflammatory marker analysis allied with acute toxicity studies in rats. The serum and tissue biochemical, histological and organ index studies of acute toxicity evaluation confirms the non-toxic nature of the extracts. The TPLE and TPFE treated groups represents complete restoration of normal skin structures after biopsy at day 14 comparable to the control group healed at day 24, the significant amount of hydroxyproline, hexosamine, hexuronic acid observed in dead space wound study, maximum wound breaking strength is intern related with the more collagen and fibroblast deposition can be correlated with the excellent wound healing efficiency of extracts. Consequently, the profound in vitro antioxidant efficacy, lowing of carrageenan induced inflammation, ESR, nitric oxide radicles and LPO (lipid peroxidation) content allied with an elevation of the GSH (reduced glutathione), SOD (superoxide dismutase), CAT (catalase), and GPx (glutathione peroxidase) in granuloma is a clear indication of good antioxidant and anti-inflammatory activity of TPLE and TPFE extracts. Our study provides evidence for the association of the excellent antioxidant, anti-inflammatory and wound healing capacity of T. peruviana present in a prudently balanced means which is required in a novel wound healing drug. Keywords : Thevetia peruviana; wound healing; antioxidant; lipid peroxidation; anti-inflammatory; nitric oxide; carrageenan