¹Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris (UPSI), 35900, Tanjong Malim, Perak, Malaysia DOI : 10.12991/jrp.2018.117 The study was conducted to investigate the effects of Aquilaria malaccensis leaves aqueous extracts on the aphrodisiac properties, which included; sexual behaviour, orientation activity, and testosterone level in ICR mice. Thirty healthy and sexually experienced male and thirty non-estrous female mice were used. In this experiment, each male was cohabitated with one female in a polysulfone cage. The mice were divided into 6 groups that received normal saline (control group), 50 mg/kg, 100 mg/kg, 200 mg/kg, 500 mg/kg, and 1000 mg/kg body weight of A. malaccensis leaves aqueous extract orally for 21 days consecutively. Results showed that all aphrodisiac parameters investigated in this study were similar between the treatment groups to the control group. However, two treated groups that received 100 mg/kg (day 14; day 21) and 200 mg/kg (day 0; day 21) resulted in significantly higher in mount frequency as compared to the control group. Overall, the results revealed that A. malaccensis leaves aqueous extract did not significantly alter the aphrodisiac parameters. Thus, this study validated that A. malaccensis leaves aqueous extracts lack of aphrodisiac properties in mice. Keywords : Aquilaria malaccensis; aphrodisiac; sexual behaviour; orientation activity; testosterone