Editor-in-Chief
Hatice Kübra Elçioğlu
Vice Editors
Levent Kabasakal
Esra Tatar
Online ISSN
2630-6344
Publisher
Marmara University
Frequency
Bimonthly (Six issues / year)
Abbreviation
J.Res.Pharm.
Former Name
Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal
2017 , Vol 21 , Issue 4
A validated HPLC method for simultaneous estimation of Melatonin and Octyl Methoxycinnamate in combined pharmaceutical applications
1Department of Pharmaceutical Technology, Faculty of Pharmacy, Ege University, Izmir, Turkey
DOI :
10.12991/mpj.2017.16
A simple, fast and precise reverse phase high performance
liquid chromatographic method has been developed for
the simultaneous determination of Melatonin and Octyl
Methoxycinnamate. Melatonin has become an attractive
substrate in sunscreen formulations because of its high
antioxidant and photo-protection properties. Octyl
methoxycinnamate is one of the chemical UV filter that can be
found most of the sunscreen formulations up to 7.5 % according
Food and Drug Administration. The aim of the present study
was to develop and validate a High-Performance Liquid
Chromatography method for the determination of Melatonin
and Octyl Methoxycinnamate in combined pharmaceutical or
cosmetic applications. As a model of combined pharmaceutical
applications, a microemulsion consisting of Melatonin and
Octyl Methoxycinnamate was also prepared and characterized
in terms of droplet size, pH and viscosity. The separation was performed with a Waters XTerra RP C18 (5 μm, 4.6 x 150 mm).
All HPLC assays were performed with 10 μl injection volume,
mobile phase consisting of acetonitrile and water, using gradient
elution starting at 20% and ending at 90% of acetonitrile with
a flow rate of 1.5 ml min-1.The eluent was monitored with
UV detection at 222 nm for Melatonin and 306 nm for Octyl
Methoxycinnamate. The method was validated according
to ICH guidelines. Validation parameters were specificity,
accuracy, precision (repeatability and reproducibility), linearity,
limit of detection (LOD) and limit of quantification (LOQ).
Analytical method development results indicated that the LOD
values were 0.132 and 0.049 μg/ml; LOQ values were 0.4 and
0.15 μg/ml and assay exhibited a linear range of 0.5- 60 μg/ml
for Melatonin and Octyl Methoxycinnamate, respectively.
Keywords :
Melatonin (PubChem CID: 896); Octyl methoxycinnamate (PubChem CID: 5355130) ; RP-HPLC method development.