¹Karadeniz Technical University, Faculty of Pharmacy, Department of Pharmacognosy, 61080 Trabzon, Turkey
²Istanbul University, Faculty of Pharmacy, Department of Pharmacognosy, 34116 Beyazıt, İstanbul, Turkey
³Karadeniz Technical University, Faculty of Arts and Sciences, Department of Chemistry, 61080 Trabzon, Turkey
⁴Karadeniz Technical University, Faculty of Pharmacy, Department of Pharmacology, 61080 Trabzon, Turkey DOI : 10.12991/marupj.300353 Volatile compounds of three Stachys species; Stachys macrantha (C. Koch) Stearn, Stachys sylvatica L. and Stachys annua ssp. annua var. annua L. were analyzed by a solid phase micro extraction (SPME) method coupled with gas chromatographyflame ionization detector (GC–FID) and gas chromatographymass spectrometry (GC-MS). A total of 38 (99.2%), 39 (99.0%) and 33 (98.8%) volatile compounds were identified from S. annua ssp. annua var. annua, S. sylvatica and S. macrantha respectively. The major volatile constituents of the investigated three Stachys species were; α-pinene (11.2%), p-cymene (18.2%), and carvacrol (28.8%) in S. macrantha, γ-muurolene (10.2%), α-cedrene (11.2%), and limonene (37.0%) in S. sylvatica and α-pinene (11,4%), β-pinene (23.1%), and (Z)-β-ocimene (24.8%) in S. annua ssp. annua var. annua. Comparison of volatile organic compounds of all three species showed that monoterpene hydrocarbons were the major constituents in S. annua ssp. annua var. annua and S. sylvatica (65.8% and 49.8% respectively), whereas oxygenated monoterpenes were the main components (42.1%) of S. macrantha. Keywords : Stachys macrantha, Stachys sylvatica, Stachys annua ssp. annua var. annua, Lamiaceae, volatile compounds, SPME