¹Celal Bayar Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muradiye, Manisa
²Manisa Hasan Türek Anadolu Lisesi, Aynıali Mahallesi Kenzi Caddesi No.24 Yunusemre, Manisa DOI : 10.12991/mpj.20162044231 In this study, a new double staining method that provide clearly to identify tissues in making preparations by hand-cut of plant samples were fixed with alcohol has been developed. This new method can be provides practicality and certainty in terms of distinguishing the tissues and can be used for hand-cut. In this method, double staining consist of safranin and fast green can be used in both monocotyledons and dicotyledons and can be stored at room temperature for a long time without spoiling. Photographs of the anatomical sections is used this method was taken and tissues was demonstrated. Keywords : Plant anatomy, Staining, Hand-cut, Fast-green, Safranin