A Fluorodensitometric method was developed for the determination of metoprolol tartrate in pharmaceutical preparations. The method was based on the reaction of metoprolol tartrate with NBD-Cl at 80o in 30 min in 0.1 M NaHCO3- MIBK two phase system and the measurement of the fluorescence intensity on silica gel plate after TLC separation. Fluorescence measurements were performed at 540 nm, using 366 nm mercury line for the excitation. The method was applied to commercially available tablets and the results were statistically compared with those obtained by HPLC method of USP XX, using t- and F- tests, t and F values were found to be 0.20 and 0.82, respectively and both lower than the table values for 95% confidence level (t=2.23 and F=5.05). Keywords :