Editor-in-Chief
Hatice Kübra Elçioğlu
Vice Editors
Levent Kabasakal
Esra Tatar
Online ISSN
2630-6344
Publisher
Marmara University
Frequency
Bimonthly (Six issues / year)
Abbreviation
J.Res.Pharm.
Former Name
Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal
2015 , Vol 19 , Issue 2
Evaluation of Antioxidant activities of Brassica napus"s seeds by CUPRAC, ABTS/Persulphate and DMPD methods
1Namik Kemal University , Art and Science Faculty, Department of Chemistry, Tekirdağ - TURKEY
DOI :
10.12991/mpj.20151910462
The antioxidant activities of the extracts prepared from Brassica
napus"s seeds (canola seeds) was determined in this study.
Brassica napus"s seeds were collected from eight different
regions of Turkey. Extraction experiments were carried out with
100% hexane, 100% acetone, 100% methanol, 100% ethanol,
%75 hexane-water, %75 acetone-water , 75% methanol-water,
75% ethanol-water, %50 hexane-water, 50% acetone-water,
50% methanol-water, 50% ethanol-water at 25 °C overnight and
were mixed by magnetic stirrer during 1h, 2h, 4h, 8h to
determine optimal extraction conditions. According to the
results of UV/VIS spectra, 100% hexane extraction managed at
25 °C overnight was chosen for working with ABTS/Persulphate, N,N-dimethyl-p-phenylenediamine (DMPD) and
CUPRAC methods. CUPRAC and ABTS/Persulphate methods"s
total antioxidant capacity (TAC) were calculated as Trolox
equivalent. In DMPD method, antioxidant capacity of the
extracts was determined by inhibition of DMPD radical cation.
DMPD method analysis results of Brassica napus"s seeds
showed the greatest effectiveness, with inhibition values of 2.00
± 0.07% and 17.1± 0.03%. The antioxidant activities of Brassica
napus"s seeds ranged from 0.29±0.04 mol/g and 0.48±0.01
mol/g for CUPRAC method and 0.36 ± 0.01 mol/g and 0.55±
0.05 mol/g for ABTS/Persulphate method.
Keywords :
Antioxidant activity, Brassica napus"s seed, ABTS/ Persulphate, CUPRAC, DMPD Methods, Trolox