Editor-in-Chief Hatice Kübra Elçioğlu Vice Editors Levent Kabasakal Esra Tatar Online ISSN 2630-6344 Publisher Marmara University Frequency Bimonthly (Six issues / year) Abbreviation J.Res.Pharm. Former Name Marmara Pharmaceutical Journal
Marmara Pharmaceutical Journal 2014 , Vol 18 , Issue 3
Protective and Therapeutic Effects of Polygonum cognatum Meissn Aqueous Extract in Experimental Colitis
Ozge CEVIK1, Azize SENER2, Zarife OZDEMIR KUMRAL3, Sule CETINEL4, Ayhan ALTINTAS5, Rabia OBA2, Berrak C. YEGEN3, Aysen YARAT6
1Department of Biochemistry, Faculty of Pharmacy, Cumhuriyet University, Turkey
2Marmara University, Faculty of Pharmacy, Department of Biochemistry, Haydarpaşa 34668 İstanbul, Turkey
3Department of Physiology, School of Medicine, Marmara University, Turkey
4Department of Histology, School of Medicine, Marmara University, Turkey
5Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Turkey
6Department of Biochemistry, Faculty of Dentistry, Marmara University, Turkey DOI : 10.12991/mpj.2014187239 The aim of the study was to investigate the protective and therapeutic effects of Polygonum cognatum Meissn (PC) in the treatment of colitis. Rats were orogastrically treated with PC (2g/kg) throughout a 10-day interval before and/or after colitis, which was induced by intracolonic administration of acetic acid. Microscopic damage scores and scanning electron microscopy changes of colon segments were analyzed. The levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in serum, malonyldialdehyde (MDA), glutathione (GSH), tissue factor (TF) levels, myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and gammaglutamyltransferase (GGT) activities in the colon tissues were measured. PC treatment ameliorated the acetic acid-induced disturbances in colonic tissue. Serum IL-6 and TNF-α levels decreased with PC treated colitis group. Colonic tissue GGT, MPO activities and MDA levels were decreased on the other hand SOD, CAT activities and GSH levels were increased in PC treated colitis group. PC was effective in the treatment of acetic acid-induced colitis in rats. This may be due to the supression and down regulation of mediators in intestinal inflammatory response and the expression of antioxidant and anti-inflammatory response. In conclusion, PC can play an protective role by downregulating the colonic tissue damage and oxidative stress which may be related to modulating the activities of antioxidant molecules in rat model of colitis. Keywords : Polygonum cognatum Meissn, Oxidative Stress, Colitis, Antioxidant, Anti-inflammatory
Marmara University