¹Marmara Üniversitesi Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye
²İstanbul Üniversitesi Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Tıbbi Genetik Bilim Dalı, İstanbul, Türkiye
³İstanbul Üniversitesi Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Nefroloji Bilim Dalı, İstanbul, Türkiye
⁴Marmara Üniversitesi Eczacılık Fakültesi, Biyokimya Anabilim Dalı, İstanbul, Türkiye DOI : 10.12991/mpj.2014187241 In our study we were investigated the in vitro effects of therapeutic concentrations of vancomycin (40 μg/ml), daptomycin (60 μg/ml), linezolid (19 μg/ml), levofloxacin (7 μg/ml), fusidic acid (36 μg/ml) and meropenem (24 μg/ml) o n t h e m a l o n d i a l d e h y d e ( M D A ) l e v e l s a n d polymorphonuclear leukocyte (PMN) functions (phagocytic and intracellular killing activity) in hemodialysis patients. PMNs were isolated from venous blood by ficoll-hypaque gradient centrifugation method. Phagocytic activity and intracellular killing activity were assayed by modified Alexander"s method. MDA level was detected by Beuge method which is as an indicator of lipid peroxidation. All results was evaluated by statisticaly. Vancomycin, daptomycin, linezolid, levofloxacin, fusidic acid and meropenem at therapeutic concentrations did not affect both PMN functions of HD patients before and after HD (p>0.05). It was observed that vancomycin, levofloxacin, fusidic acid and meropenem at therapeutic concentrations increased PMN"s MDA levels of HD patients before HD but decreased MDA levels after HD (p<0.05). Daptomycin and linezolid did not change PMN"s MDA levels of HD patients before and after HD (p>0.05). Keywords : Hemodialysis, oxidative stress, polymorphonuclear leukocyte functions