¹Ege University, Faculty of Pharmacy, Department of Radiopharmacy, İzmir, Turkey
²Ege University, Faculty of Medicine, Department of Nuclear Medicine, İzmir, Turkey
³Ege University, Faculty of Pharmacy, Department of Pharmaceutical Technology, İzmir, Turkey DOI : 10.12991/2013171310 The aim of this study was to compare the bone uptake of alendronate sodium (ALD) from vaginal suppositories prepared with massa estarinum AB (ME) and polyethylene glycol 1500 (PEG) bases. For this purpose, ALD was radiolabeled with ^99mTecnetium Pertechnetate (^99mTc) by direct method. Radiochemical purity and stability of ^99mTc-ALD was performed with chromatographic studies. ^99mTc-ALD containing suppositories were prepared with ME and PEG bases. Physical properties of suppositories were evaluated. The physicochemical diffusion study was carried out to compare the release of ALD from different suppository bases. The bone uptake of ^99mTc-ALD was observed by gamma scintigraphy studies. ^99mTc-ALD containing suppositories were administrated to rabbits via vaginal route. The scintigraphic images were obtained with a gamma camera at different time intervals up to 240 minutes. According to our studies, radiochemical purity of ^99mTc-ALD was observed more than 95% up to 6 hours. At 240 minutes of physicochemical diffusion studies, released ALD has 0.620 ± 0.091 mm and 10.465 ± 0.651 mm diameter zone from ME and PEG base suppositories respectively. According to the gamma scintigraphy studies, although no bone uptake observed after ME suppositories application, rabbit's bones were clearly visible after PEG suppositories applied. The results of physicochemical diffusion and gamma scintigraphy studies were found compatible in each other. Keywords : Alendronate sodium, bone uptake, vaginal suppository, massa estarinum, polyethylene glycol