Department of Biopharmaceutics and Pharmacokinetics, Ege University, Faculty of Pharmacy, Izmir, Turkey DOI : 10.12991/2013171309 In this study, the potential use of thermotropic liquid crystals as a responsive release system was investigated. Cholesteryl oleyl carbonate liquid crystal (COC) has been embedded in nylon membranes by using vacuum filtration methods. In vitro drug release studies were performed by using a fluid/fluid diffusion cell. Sodium salicylate as a model drug penetration study was performed in nylon membrane with or without COC that is prepared at 25 and 37°C. Also the penetration studies were performed by repeatedly exchanging the temperature cycle (10, 25, 37°C) of the water bath at predetermined intervals. It was observed that COC and temperature have effects on the penetration of sodium salicylate. At 37 °C, the penetration rate of sodium salicylate was found higher than 25 °C and 10 °C, respectively. Also, the penetration amount of sodium salicylate has changed as pulsatile drug delivery in the presence of COC. As a result, according to the patients' requirements, it seems that the penetration rate and profile can be adjusted by developing pulsatile drug delivery as a new system. Keywords : Cholesteryl oleyl carbonate, nylon membrane, pulsatile drug delivery, sodium salicylate